Recombinant Human VTCN1 protein, His-tagged
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
|Product Overview :||Recombinant Human VTCN1 (Phe29-Ala258) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||V-set domain-containing T-cell activation inhibitor 1 (VTCN1) is also known as Immune costimulatory protein B7-H4, Protein B7S1, T-cell costimulatory molecule B7x, B7H4, which belongs to the immunoglobulin superfamily and BTN/MOG family. VTCN1 contains two Ig-like V-type (immunoglobulin-like) domains. The expression of VTCN1 is up-regulated by IL6 and IL10 and is inhibited by GM-CSF and IL4 on antigen-presenting cells (APCs). VTCN1/B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. VTCN1 involved in promoting epithelial cell transformation.|
|Predicted N Terminal :||Phe29|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 26.1 kDa. The protein migrates as 43-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Phe29-Ala258|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of VTCN1-662H :||
Human B7-H4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of VTCN1-662H :||
Immobilized Human B7-H4, His Tag at 2 μg/mL (100 μL/well) can bind Anti-B7-H4 MAb (Human IgG1) with a linear range of 0.2-6 ng/mL (QC tested).
Batch consistency of Bioactivity-ELISA
|Gene Name :||VTCN1|
|Official Symbol :||VTCN1|
|Synonyms :||VTCN1; V-set domain containing T cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; B7 family member; H4; B7 superfamily member 1; B7 H4; B7H4; B7S1; B7X; FLJ22418; B7 family member, H4; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4; B7-H4; B7h.5; VCTN1; PRO1291; RP11-229A19.4|
|Gene ID :||79679|
|mRNA Refseq :||NM_001253849|
|Protein Refseq :||NP_001240778|
|UniProt ID :||Q7Z7D3|
◆ Recombinant Protein
|VTCN1-787M||Recombinant Mouse VTCN1 Protein (Phe29-Ser256), His-tagged||+Inquiry|
|VTCN1-333H||Recombinant Human VTCN1 Protein, His & Avi-tagged, Biotinylated||+Inquiry|
|VTCN1-430H||Recombinant Human VTCN1 Protein (Phe29-Ala258), HIgG1 Fc-tagged, Biotinylated||+Inquiry|
|VTCN1-788M||Recombinant Mouse VTCN1 Protein (Phe29-Ser256), His-tagged||+Inquiry|
|VTCN1-429H||Recombinant Human VTCN1 Protein, His-tagged, Biotinylated||+Inquiry|
|VTCN1-1096HCL||Recombinant Human VTCN1 cell lysate||+Inquiry|
|VTCN1-1636MCL||Recombinant Mouse VTCN1 cell lysate||+Inquiry|