Recombinant Human WARS, His-tagged

• Cat.No.: WARS-30746TH
• Product Overview: Recombinant full length Human Tryptophanyl tRNA synthetase , with N terminal His-tag; 491 amino acids with predicted MWt 55.3kDa inclusive of tag

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 471 amino acids
• Description: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.
• Conjugation: HIS
• Molecular Weight: 55.300kDa inclusive of tags
• Form: Liquid
• Purity: >90% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQIKTKVNKHAFSGGRDTIEEHRQFGGNCDVDVSFMYLTFFLEDDDKLEQIRKDYTSGAMLTGELKKALIEVLQPLIAEHQARRKEVTDEIVKEFMTPRKLSFDFQ
• Sequence Similarities: Belongs to the class-I aminoacyl-tRNA synthetase family.Contains 1 WHEP-TRS domain.

Gene Information

• Gene Name: WARS tryptophanyl-tRNA synthetase [ Homo sapiens ]
• Official Symbol: WARS
• Synonyms: WARS; tryptophanyl-tRNA synthetase; IFI53; tryptophanyl-tRNA synthetase, cytoplasmic; IFP53; tryptophan tRNA ligase 1; cytoplasmic
• Gene ID: 7453
• mRNA Refseq: NM_004184
• Protein Refseq: NP_004175
• MIM: 191050
• Uniprot ID: P23381
• Chromosome Location: 14q32.2
• Pathway: Aminoacyl-tRNA biosynthesis, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, conserved biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, organism-specific biosystem; Aminoacyl-tRNA biosynthesis, eukaryotes, conserved biosystem; Cytosolic tRNA aminoacylation, organism-specific biosystem
• Function: ATP binding; aminoacyl-tRNA ligase activity; ligase activity; nucleotide binding; protein binding