Recombinant Human WEE1 Protein (215-646, end), N-FLAG tagged

• Cat.No.: WEE1-14H
• Product Overview: Human Wee1 protein, also known as Wee1A Kinase, GenBank Accession No. NM_003390, amino acids 215-646 (end), with an N-terminal FLAG-tag, expressed in a Baculovirus infected Sf9 cell expression system.

Specification

• Description: This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.
• Source: Insect cells
• Species: Human
• Tag: N-FLAG
• Form: Aqueous buffer solution
• Molecular Mass: 50 kDa
• Protein Length: 215-646
• Storage: At least 6 months at –80 centigrade.
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 100 μg/mL FLAG peptide and 20 % Glycerol.
• Shipping: At -80 centigrade
• Warning: Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 μg/ml in PBS + glycerol and stored at -80 centigrade.

Gene Information

• Gene Name: WEE1 WEE1 G2 checkpoint kinase [ Homo sapiens (human) ]
• Official Symbol: WEE1
• Synonyms: WEE1; WEE1 G2 checkpoint kinase; WEE1A; WEE1hu; wee1-like protein kinase; WEE1 homolog; WEE1+ homolog; protein kinase; wee1A kinase; EC 2.7.10.2
• Gene ID: 7465
• mRNA Refseq: NM_003390
• Protein Refseq: NP_003381
• MIM: 193525
• UniProt ID: P30291