| Description : |
Oocyte-specific protein tyrosine kinase that phosphorylates and inhibits CDK1/CDC2 and acts as a key regulator of meiosis during both prophase I and metaphase II. Required to maintain meiotic arrest in oocytes during the germinal vesicle (GV) stage, a long period of quiescence at dictyate prophase I, by phosphorylating CDK1 at 'Tyr-15', leading to inhibit CDK1 activity and prevent meiotic reentry. Also required for metaphase II exit during egg activation by phosphorylating CDK1 at 'Tyr-15', to ensure exit from meiosis in oocytes and promote pronuclear formation. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
62.7 kDa |
| AA Sequence : |
MDDKDIDKELRQKLNFSYCEETEIE GQKKVEESREASSQTPEKGEVQDSE AKGTPPWTPLSNVHELDTSSEKDKE SPDQILRTPVSHPLKCPETPAQPDS RSKLLPSDSPSTPKTMLSRLVISPT GKLPSRGPKHLKLTPAPLKDEMTSL ALVNINPFTPESYKKLFLQSGGKRK IRGDLEEAGPEEGKGGLPAKRCVLR ETNMASRYEKEFLEVEKIGVGEFGT VYKCIKRLDGCVYAIKRSMKTFTEL SNENSALHEVYAHAVLGHHPHVVRY YSSWAEDDHMIIQNEYCNGGSLQAA ISENTKSGNHFEEPKLKDILLQISL GLNYIHNSSMVHLDIKPSNIFICHK MQSESSGVIEEVENEADWFLSANVM YKIGDLGHATSINKPKVEEGDSRFL ANEILQEDYRHLPKADIFALGLTIA VAAGAESLPTNGAAWHHIRKGNFPD VPQELSESFSSLLKNMIQPDAEQRP SAAALARNTVLRPSLGKTEELQQQL NLEKFKTATLERELREAQQAQSPQG YTHHGDTGVSGTHTGSRSTKRLVGG KSARSSSFTSGEREPLHTRTRPLEQ KLISEEDLAANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
