Active Recombinant Human WISP1 protein, His-tagged

• Cat.No.: WISP1-2332H
• Product Overview: Active Recombinant Human WISP1 protein(NP_003873.1) (Met 1-Asn 367) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-367 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured in a cell proliferation assay using NRK-49F rat fibroblasts (Xu, L. et al., 2000, Gene and Dev. 14: 585.). The ED50 for this effect is typically 10-25 μg/ml.
• Molecular Mass: The secreted recombinant human WISP1 consisting of 356 amino acids has a calculated molecular mass of 39 kDa. As a result of glycosylation, It migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: WISP1 WNT1 inducible signaling pathway protein 1 [ Homo sapiens ]
• Official Symbol: WISP1
• Synonyms: WISP1; WNT1 inducible signaling pathway protein 1; WNT1-inducible-signaling pathway protein 1; CCN4; CCN family member 4; WNT1 induced secreted protein 1; Wnt-1 inducible signaling pathway protein 1; WISP1c; WISP1i; WISP1tc; FLJ14388
• Gene ID: 8840
• mRNA Refseq: NM_001204869
• Protein Refseq: NP_001191798
• MIM: 603398
• UniProt ID: O95388