Recombinant Human WNK4 Protein (L2-M1243), His/GST tagged

• Cat.No.: WNK4-1099H
• Product Overview: Recombinant Human 8His-GST-TEV-GG-WNK4(L2-M1243 end) Protein was expressed in Insect cell.

Specification

• Description: Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
• Source: Insect cell
• Species: Human
• Tag: His/GST
• Form: Liquid
• Protein Length: L2-M1243
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: WNK4 putative protein kinase WNK4 [ Homo sapiens (human) ]
• Official Symbol: WNK4
• Synonyms: WNK4; putative protein kinase WNK4
• Gene ID: 65266
• mRNA Refseq: NM_032387
• Protein Refseq: NP_115763
• MIM: 601844
• UniProt ID: Q96J92