Recombinant Human XBP1 Protein, GST-HIS-tagged

• Cat.No.: XBP1-092H
• Product Overview: Recombinant Human XBP1, transcript variant 1, fused with N-terminall GST and C-terminall His was expressed in E. coli.

Specification

• Description: This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
• Source: E. coli
• Species: Human
• Tag: GST-His
• Form: 25mM Tris, pH8.0, 150mM NaCl, 10% glycerol, 1% Sarkosyl.
• Molecular Mass: 54.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: XBP1 X-box binding protein 1 [ Homo sapiens ]
• Official Symbol: XBP1
• Synonyms: XBP1; X-box binding protein 1; XBP2; X-box-binding protein 1; tax-responsive element-binding protein 5; TREB5; XBP-1
• Gene ID: 7494
• mRNA Refseq: NM_001079539
• Protein Refseq: NP_001073007
• MIM: 194355
• UniProt ID: P17861