Recombinant Human XIAP Protein (Y120-P260), GST tagged

• Cat.No.: XIAP-0712H
• Product Overview: Recombinant Human GST-Thrombin-GG-XIAP(Y120-P260) Protein was expressed in E. coli.

Specification

• Description: Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, CASP3, CASP7, CASP8, CASP9, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS and BIRC5/survivin. Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinating COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Ubiquitinates and therefore mediates the proteosomal degradation of BCL2 in response to apoptosis. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program.
• Source: E. coli
• Species: Human
• Tag: GST
• Form: Liquid
• Protein Length: Y120-P260
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 85%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50mM Tris-HCl (pH 7.5), 200mM NaCl, 5% glycerol, 1mM DTT.
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: XIAP X-linked inhibitor of apoptosis [ Homo sapiens (human) ]
• Official Symbol: XIAP
• Synonyms: XIAP; X-linked inhibitor of apoptosis; API3, baculoviral IAP repeat containing 4, BIRC4; baculoviral IAP repeat-containing protein 4; hILP; X-linked IAP; IAP-like protein; E3 ubiquitin-protein ligase XIAP; inhibitor of apoptosis protein 3; baculoviral IAP repeat-containing 4; X-linked inhibitor of apoptosis protein; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3; FLJ26913
• Gene ID: 331
• mRNA Refseq: NM_001167
• Protein Refseq: NP_001158
• MIM: 300079
• UniProt ID: P98170