Recombinant Human XRCC1 Protein, His-tagged

• Cat.No.: XRCC1-596H
• Product Overview: Recombinant Human XRCC1 Protein (Met1-Ser219) with N-His tag was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: Met1-Ser219
• Description: The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 27.5 kDa
  Accurate Molecular Mass: 28&33 kDa
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1 mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: XRCC1 X-ray repair complementing defective repair in Chinese hamster cells 1 [ Homo sapiens (human) ]
• Official Symbol: XRCC1
• Synonyms: XRCC1; X-ray repair complementing defective repair in Chinese hamster cells 1; RCC; DNA repair protein XRCC1; X-ray repair cross-complementing protein 1; X-ray-repair, complementing defective, repair in Chinese hamster
• Gene ID: 7515
• mRNA Refseq: NM_006297
• Protein Refseq: NP_006288
• MIM: 194360
• UniProt ID: P18887