Recombinant Influenza A H1N1 (A/Beijing/22808/2009) HA protein, His-tagged

• Cat.No.: HA-0358H
• Product Overview: Recombinant Influenza A H1N1 (A/Beijing/22808/2009) HA protein(Met1-Gln529)(ADD64203.1), fused with His tag, was expressed in HEK293.

Specification

• Species: Influenza A H1N1 (A/Beijing/22808/2009)
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gln529
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The secreted recombinant hemagglutinin of H1N1(A/Beijing/22808/2009)HA (A/Beijing/22808/2009 (H1N1)) comprises 523 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 72 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.