Species : |
Influenza A H1N1 (A/Wisconsin/67/2022) |
Source : |
HEK293 |
Tag : |
His |
Form : |
Lyophilized from sterile PBS, pH 7.4. |
Bio-activity : |
Immobilized Anti-H1N1 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Influenza A H1N1 (A/Wisconsin/67/2022) HA Trimer Protein (His Tag), the EC50 is 1.5-5 ng/mL. |
Molecular Mass : |
The recombinant Influenza A virus ((A/Wisconsin/67/2022) (H1N1))Hemagglutinin / HA comprises 521 amino acids and has a predicted molecular mass of 58.79 kDa. It migrates as an approximately 74.5 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 167.3 kDa verified by SEC-MALS. |
Endotoxin : |
< 1.0 EU per μg protein as determined by the LAL method. |
Purity : |
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS. |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |