Recombinant H1N1(A/Puerto Rico/8/34/Mount Sinai) NP protien, His-tagged

• Cat.No.: NP-3339I
• Product Overview: Recombinant H1N1(A/Puerto Rico/8/34/Mount Sinai) NP protien(AAM75159.1) (Met1-Gly490) was expressed in Insect Cells, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: H1N1
• Source: Insect Cells
• Tag: His
• Protein Length: 1-490 aa
• Form: Lyophilized from sterile 60mM Tris, 500mM NaCl, pH 7.4.
• Molecular Mass: The recombinant Influenza A virus (A/Puerto Rico/8/34/Mount Sinai (H1N1)) nucleoprotein comprises 501 amino acids and has a predicted molecular mass of 56.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.