Recombinant Influenza A H3N2 (A/District of Columbia/27/2023) HA protein, His-tagged

• Cat.No.: HA-7332V
• Product Overview: Recombinant Influenza A H3N2 (A/District of Columbia/27/2023) HA protein(translated amino acids of EPI2990337)(Met1-Val521), was expressed in HEK293 with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.

Specification

• Species: Influenza A H3N2 (A/District of Columbia/27/2023)
• Source: HEK293
• Tag: His
• Protein Length: 1-521 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: 1.Immobilized Recombinant Influenza A H3N2 HA Protein (His Tag) at 2 μg/mL (100 μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody, the EC50 is 8-25 ng/mL. 2.Immobilized Recombinant Influenza A H3N2 HA Protein (His Tag) at 2 μg/mL (100 μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody, the EC50 is 5-15 ng/mL.
• Molecular Mass: The recombinant Influenza A virus (A/District of Columbia/27/2023 (H3N2)) hemagglutinin comprises 558 amino acids and has a predicted molecular mass of 62.41 kDa. It migrates as approximately 83.6 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.