| Species : |
Influenza A H3N2 (A/District of Columbia/27/2023) |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
1-521 aa |
| Form : |
Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80. |
| Bio-activity : |
1.Immobilized Recombinant Influenza A H3N2 HA Protein (His Tag) at 2 μg/mL (100 μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody, the EC50 is 8-25 ng/mL.
2.Immobilized Recombinant Influenza A H3N2 HA Protein (His Tag) at 2 μg/mL (100 μL/well) can bind a broadly neutralizing influenza HA stem-specific antibody, the EC50 is 5-15 ng/mL. |
| Molecular Mass : |
The recombinant Influenza A virus (A/District of Columbia/27/2023 (H3N2)) hemagglutinin comprises 558 amino acids and has a predicted molecular mass of 62.41 kDa. It migrates as approximately 83.6 kDa band in SDS-PAGE under reducing conditions. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
> 95 % as determined by SDS-PAGE. |
| Storage : |
Store it under sterile conditions at -20 to -80 ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents. |
