| Species : |
H5N1 |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
1-531 aa |
| Form : |
Lyophilized from sterile PBS, pH 7.4. |
| Bio-activity : |
Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.3-1.5 μg/mL for 1% GRBC.
It also agglutinates chick red blood cells. |
| Molecular Mass : |
The secreted recombinant hemagglutinin of Influenza A virus H5N1 (A/Indonesia/5/2005) comprises 526 amino acids with the predicted molecular mass of 60 kDa. As a result of glycosylation, it migrates as an approximately 65-70 kDa band in Non-reduced SDS-PAGE, and three bands (25, 45, 70 kDa) in reduced SDS-PAGE. |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
| Purity : |
> 95 % as determined by SDS-PAGE |
| Storage : |
Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
