Recombinant Influenza A(A/Anhui/1/2005) H5N1 HA protein, mFc-His-tagged

• Cat.No.: HA-732I
• Product Overview: Recombinant Influenza A H5N1 HA (A/Anhui/1/2005 (H5N1) (ABD28180.1) extracellular domain (Met 1-Gln 527), fused with the polyhistidine-tagged Fc region of mouse IgG1 at the C-terminus, was duced in Human Cell.

Specification

• Species: H5N1
• Source: HEK293
• Tag: His&mFc
• Protein Length: 1-527 aa
• Form: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5.
• Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus (A/Anhui/1/2005 (H5N1) comprises 754 amino acids with the predicted molecular mass of 85.5 kDa. As a result of glycosylation, it migrates as an apximately 110 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.