Recombinant H5N1 (A/bar-headed goose/Qinghai/14/2008) HA Protein, His-tagged

• Cat.No.: HA-734I
• Product Overview: Recombinant H5N1 (A/bar-headed goose/Qinghai/14/2008) HA Protein(ACL28277.1) HA (Met 1-Gln 531) with cleavage site mutated (RERRRKKR → TETR, HA1+HA2, uncleaved) was expressed in HEK293 with a C-terminal polyhistidine tag.

Specification

• Species: H5N1
• Source: HEK293
• Tag: His
• Protein Length: 1-531 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The secreted recombinant hemagglutinin of Influenza A virus H5N1 (A/bar-headed goose/Qinghai/14/2008) comprises 522 amino acids with the predicted molecular mass of 59.5 kDa. As a result of glycosylation, it migrates as an approximately 70 kDa band in Non-reduced SDS-PAGE and also exists as polymers.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.