Recombinant MERS-CoV N Protein (Met1-Thr411), C-6×His-tagged
Cat.No. : | N-01M |
Product Overview : | Recombinant MERS-CoV N Protein (Met1-Thr411) with a C-terminal 6-His tag was expressed in Spodoptera frugiperda, Sf 21 (baculovirus). |
- Specification
- Gene Information
- Related Products
Description : | MERS-CoV, which causes the Middles East Respiratory Syndrome (MERS), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N). While the S, E and M proteins build up the viral envelope, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP). The MERS-CoV N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an Intrinsically Disordered Region and an RNA binding domain, the linker region interacts with the M protein and the C-terminal region contains a self-association domain. The MERS-CoV N protein shares 46.3% and 4.5% amino acid sequence identity with SARS-CoV-1 and SARS-CoV-2 N protein, respectively. MERS-CoV N proteins have been shown to inhibit Type I Interferon(IFN) production. In addition, the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity, making it a promising therapeutic target. |
Source : | Insect cell |
Species : | MERS-CoV |
Tag : | C-6×His |
Bio-activity : | Bioassay data are not available. |
Molecular Mass : | Predicted Molecular Mass: 46 kDa SDS-PAGE: 49-55 kDa, under reducing conditions |
Protein Length : | Met1-Thr411 |
N-terminal Sequence Analysis : | Sequence has been confirmed by mass spectrometry |
Endotoxin : | <0.10 EU/μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Storage Buffer : | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution : | Reconstitute at 100 μg/mL in PBS. |
Shipping : | The product is shipped at ambient temperature. |
Gene Name : | N nucleocapsid protein [ Middle East respiratory syndrome-related coronavirus ] |
Official Symbol : | N |
Synonyms : | N; nucleocapsid protein; nucleocapsid protein; nucleoprotein |
Gene ID : | 14254601 |
Protein Refseq : | YP_009047211 |
UniProt ID : | K0BVN3 |
Products Types
◆ Lysates | ||
NP-002HCL | Recombinant H3N2 NP cell lysate | +Inquiry |
NP-001HCL | Recombinant H1N1 NP cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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