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Recombinant Mouse Agxt Protein, MYC/DDK-tagged

Cat.No. : Agxt-555M
Product Overview : Purified recombinant protein of full-length mouse alanine-glyoxylate aminotransferase (Agxt), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
  • Specification
  • Gene Information
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Description : Dual metabolic roles of gluconeogenesis (in the mitochondria) and glyoxylate detoxification (in the peroxisomes).
Source : HEK293T
Species : Mouse
Tag : MYC/DDK
Molecular Mass : 45.9 kDa
Purity : >80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Agxt alanine-glyoxylate aminotransferase [ Mus musculus (house mouse) ]
Official Symbol : Agxt
Synonyms : Agxt; alanine-glyoxylate aminotransferase; AGT; SPT; Agt1; Agxt1; serine--pyruvate aminotransferase, mitochondrial; serine--pyruvate aminotransferase, peroxisomal; alanine-glyoxylate aminotransferase 1; serine-pyruvate aminotransferase; EC 2.6.1.44; EC 2.6.1.51
Gene ID : 11611
mRNA Refseq : NM_016702
Protein Refseq : NP_057911
UniProt ID : O35423

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Are there any known structural motifs in AGXT essential for its enzymatic activity? 02/28/2022

AGXT contains a PLP-binding domain, characteristic of aminotransferases, which is critical for its enzymatic activity and substrate specificity.

Can mutations in the AGXT gene lead to any hereditary disorders? 06/04/2021

Yes, mutations in the AGXT gene can cause primary hyperoxaluria type 1 (PH1), a rare autosomal recessive disorder characterized by the overproduction and accumulation of oxalate, resulting in severe kidney and urinary tract complications.

Can AGXT be targeted therapeutically for the treatment of kidney stones in non-PH1 patients? 01/07/2021

Targeting AGXT enzymatic activity may hold promise for developing therapies to prevent kidney stone formation in non-PH1 patients; however, further research is needed to validate this approach.

Which cellular compartment is AGXT predominantly localized in? 12/30/2020

AGXT is primarily localized in the peroxisomes, a subcellular organelle responsible for various metabolic processes, including lipid metabolism and detoxification.

Can AGXT activity be influenced by epigenetic modifications? 10/28/2020

Epigenetic modifications, such as DNA methylation or histone acetylation, could potentially regulate AGXT gene expression and activity, affecting oxalate metabolism.

How is AGXT activity regulated at the post-translational level? 03/15/2020

AGXT activity can be regulated by phosphorylation, allosteric modulation, and interaction with other proteins involved in peroxisomal metabolism.

What is the primary function of AGXT in the human body? 08/17/2019

AGXT plays a crucial role in the peroxisomal glyoxylate pathway, converting glyoxylate to glycine to prevent the accumulation of harmful oxalate, which can lead to kidney stone formation.

How does AGXT deficiency lead to hyperoxaluria in PH1 patients? 05/23/2019

In PH1 patients, AGXT deficiency impairs the conversion of glyoxylate to glycine, leading to the accumulation of glyoxylate. Excessive glyoxylate is converted to oxalate, which can form kidney stones and cause tissue damage.

Is AGXT involved in other physiological processes beyond oxalate metabolism? 12/19/2018

AGXT may participate in other cellular processes or metabolic pathways, and its broader functional roles warrant further exploration.

What is the reaction catalyzed by AGXT in the glyoxylate pathway? 11/10/2018

AGXT catalyzes the conversion of glyoxylate and L-alanine to glycine and pyruvate, utilizing PLP as a cofactor.

Customer Reviews (5)

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Reviews
03/07/2022

    The customer service was fantastic—helpful, friendly, and made the ordering process a breeze!

    02/10/2022

      The efficiency of this protein is outstanding. Saved me a lot of time!

      07/30/2021

        I never knew how much easier my work could be until I tried this protein.

        11/16/2019

          I'm so grateful for this protein – it's a game-changer for my studies!

          08/02/2018

            I can confidently say that this product is worth every penny.

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