Recombinant Mouse Ahcy Protein, His-tagged

• Cat.No.: Ahcy-01M
• Product Overview: Recombinant mouse Ahcy (1-432aa), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: Enables adenosylhomocysteinase activity; copper ion binding activity; and protein self-association. Acts upstream of or within S-adenosylhomocysteine catabolic process. Located in cytosol. Is expressed in several structures, including alimentary system; integumental system; nervous system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in hypermethioninemia with deficiency of S-adenosylhomocysteine hydrolase. Orthologous to human AHCY (adenosylhomocysteinase).
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid
• Molecular Mass: 50.2 kDa (456aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMGSHM SDKLPYKVADIGLAAWGRKALDIAE NEMPGLMRMREMYSASKPLKGARIA GCLHMTVETAVLIETLVALGAEVRW SSCNIFSTQDHAAAAIAKAGIPVFA WKGETDEEYLWCIEQTLHFKDGPLN MILDDGGDLTNLIHTKYPQLLSGIR GISEETTTGVHNLYKMMSNGILKVP AINVNDSVTKSKFDNLYGCRESLID GIKRATDVMIAGKVAVVAGYGDVGK GCAQALRGFGARVIITEIDPINALQ AAMEGYEVTTMDEACKEGNIFVTTT GCVDIILGRHFEQMKDDAIVCNIGH FDVEIDVKWLNENAVEKVNIKPQVD RYWLKNGRRIILLAEGRLVNLGCAM GHPSFVMSNSFTNQVMAQIELWTHP DKYPVGVHFLPKKLDEAVAEAHLGK LNVKLTKLTEKQAQYLGMPINGPFK PDHYRY
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: 20mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.2M NaCl, 1mM DTT

Gene Information

• Gene Name: Ahcy S-adenosylhomocysteine hydrolase [ Mus musculus (house mouse) ]
• Official Symbol: Ahcy
• Synonyms: Ahcy; S-adenosylhomocysteine hydrolase; CuBP; SAHH; AA987153; AL024110; adenosylhomocysteinase; S-adenosyl-L-homocysteine; hydrolase; adoHcyase; liver copper-binding protein; EC 3.3.1.1
• Gene ID: 269378
• mRNA Refseq: NM_016661
• Protein Refseq: NP_057870
• UniProt ID: P50247