Recombinant Mouse AMICA1 cell lysate

Cat.No. : AMICA1-001MCL
Product Overview : Mouse AMICA1 / JAML derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.
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Source : Human cells
Species : Mouse
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Tag : Non
Gene Name : Amica1 adhesion molecule, interacts with CXADR antigen 1 [ Mus musculus ]
Official Symbol : AMICA1
Synonyms : AMICA1; adhesion molecule, interacts with CXADR antigen 1; junctional adhesion molecule-like; mCrea7; adhesion molecule AMICA; dendritic cell-specific protein CREA7; dendritic-cell specific protein Crea7; AMICA; Crea7; Gm638; MGC61025;
Gene ID : 270152
mRNA Refseq : NM_001005421
Protein Refseq : NP_001005421
Pathway : Adaptive Immune System, organism-specific biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem; Immune System, organism-specific biosystem; Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem; Integrin cell surface interactions, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
Function : cell adhesion molecule binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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02/26/2020

    Compatibility with complex biological matrices for translational research.

    07/24/2019

      Enhanced assay sensitivity for detection of subtle protein changes.

      06/30/2018

        Accurate determination of protein function and activity.

        Q&As (10)

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        What are the downstream signaling pathways activated by AMICA1 and their functional consequences? 12/19/2022

        Activation of AMICA1 triggers downstream signaling pathways, such as Rho GTPases, PI3K/AKT, or MAPK/ERK, which modulate cytoskeletal rearrangement, cell survival, and gene expression, impacting cellular functions.

        How does AMICA1 contribute to the formation and maintenance of tissue architecture? 02/10/2022

        AMICA1 contributes to the formation and maintenance of tissue architecture by promoting cell adhesion, regulating cell polarity, and facilitating tissue-specific cellular organization.

        What is the tissue-specific expression pattern of AMICA1 in different organs? 11/29/2021

        AMICA1 exhibits a tissue-specific expression pattern, with higher expression observed in epithelial tissues, neural tissues, and certain organs like the liver and kidney.

        Can targeting AMICA1 be a potential therapeutic strategy for diseases involving abnormal cell adhesion? 08/28/2020

        Targeting AMICA1 signaling or interaction with specific antibodies or small molecules holds potential as a therapeutic strategy for diseases associated with abnormal cell adhesion or tissue integrity.

        How does AMICA1 interact with other cell adhesion molecules and extracellular matrix components? 08/26/2020

        AMICA1 interacts with various cell adhesion molecules, such as cadherins, integrins, or selectins, as well as extracellular matrix components like collagen or fibronectin, facilitating cell-cell and cell-matrix interactions.

        Does AMICA1 have post-translational modifications that impact its function or stability? 02/22/2020

        AMICA1 can undergo post-translational modifications, including phosphorylation, glycosylation, or ubiquitination, which can affect its adhesive properties, intracellular signaling, or protein stability.

        How does AMICA1 expression change during development and tissue regeneration? 12/16/2018

        During development and tissue regeneration, AMICA1 expression is dynamically regulated, showing distinct spatiotemporal patterns that coincide with tissue remodeling and organogenesis.

        What is the role of AMICA1 in cell-cell adhesion and cell migration processes? 08/19/2018

        AMICA1 plays a crucial role in cell-cell adhesion and cell migration processes, promoting the formation of stable intercellular junctions and regulating cellular movement and tissue morphogenesis.

        What is the impact of AMICA1 dysregulation on disease progression or pathological conditions? 12/30/2017

        Dysregulation of AMICA1 expression or function has been implicated in various diseases, including cancer metastasis, epithelial barrier dysfunction, or neurodevelopmental disorders, underscoring its involvement in disease pathogenesis.

        Can modulation of AMICA1 expression or activity influence cellular phenotypes, such as cell proliferation or differentiation? 07/17/2017

        Modulation of AMICA1 expression or activity can influence cellular phenotypes, including cell proliferation, differentiation, and cell fate determination, highlighting its role in tissue homeostasis and development.

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