Recombinant Mouse Aplf Protein, Myc/DDK-tagged

• Cat.No.: Aplf-1661M
• Product Overview: Purified recombinant protein of mouse full-length aprataxin and PNKP like factor (Aplf), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ).
• Molecular Mass: 55.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Aplf aprataxin and PNKP like factor [ Mus musculus (house mouse) ]
• Official Symbol: Aplf
• Synonyms: APLF; aprataxin and PNKP like factor; aprataxin and PNK-like factor; apurinic-apyrimidinic endonuclease APLF; AI452191; 2010301N04Rik
• Gene ID: 72103
• mRNA Refseq: NM_001170489
• Protein Refseq: NP_001163960
• UniProt ID: Q9D842