Recombinant Mouse Apoe Protein, MYC/DDK-tagged

• Cat.No.: Apoe-820M
• Product Overview: Purified recombinant protein of full-length mouse apolipoprotein E (Apoe), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.

Specification

• Description: This gene encodes a member of the apolipoprotein A1/A4/E family of proteins. This protein is involved in the transport of lipoproteins in the blood. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. Homozygous knockout mice for this gene accumulate high levels of cholesterol in the blood and develop atherosclerosis. Different alleles of this gene have been associated with either increased risk or a protective effect for Alzheimer's disease in human patients. This gene maps to chromosome 7 in a cluster with the related apolipoprotein C1, C2 and C4 genes.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 36.3 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Apoe apolipoprotein E [ Mus musculus (house mouse) ]
• Official Symbol: Apoe
• Synonyms: Apoe; apolipoprotein E; Apo-E; AI255918; apolipoprotein E
• Gene ID: 11816
• mRNA Refseq: NM_009696
• Protein Refseq: NP_033826
• UniProt ID: P08226

Reviews

08/28/2021

By using APOE protein, I can gain insights into the underlying molecular processes contributing to amyloidosis, which can ultimately aid in the development of novel therapeutic strategies.

02/17/2019

The manufacturer plays a crucial role in supporting my research with their extensive expertise and resources.

01/26/2018

This assurance enables me to confidently utilize the APOE protein in my research, knowing that it will consistently deliver reliable and accurate results.

10/30/2016

the manufacturer facilitates access to the latest scientific advancements and developments related to APOE protein research.

Q&As

Can APOE gene editing or gene therapy be a potential treatment option in the future?

Gene editing or gene therapy targeting the APOE gene is an area of active research. Many scientific advancements have been made in the field of gene editing, such as CRISPR-Cas9 technology, which allows for precise modifications to genes. However, gene editing and gene therapy approaches for APOE are still in the experimental stage and require further refinement and study before they can become viable treatment options.

Does the APOE protein influence mental health conditions like depression or anxiety?

While the APOE protein is primarily associated with lipid metabolism and neurodegenerative diseases, emerging evidence suggests it may play a role in mental health. Some studies have found an association between the APOEε4 allele and increased risk of developing late-onset depression or anxiety disorders. However, more research is needed to fully understand the relationship between APOE and mental health conditions.

Can medications or supplements influence the expression or function of the APOE protein?

Some medications and supplements have been studied for their potential to influence the expression or function of the APOE protein. For example, statins, commonly prescribed for high cholesterol, have shown some effects on APOE expression and metabolism. However, more research is needed to fully understand the impact of medications and supplements on APOE function.

Can genetic testing determine an individual's APOE genotype?

Yes, genetic testing can determine an individual's APOE genotype. Through a simple blood or saliva sample, the presence of APOE variants (ε2, ε3, ε4) can be detected and analyzed. It is important to note that genetic testing should be done under the supervision of a healthcare professional, as test results need to be interpreted carefully, considering various factors.

Are there any dietary changes that can optimize APOE function?

Certain dietary changes can help optimize APOE function. Consuming a diet rich in fruits, vegetables, whole grains, lean proteins, and healthy fats can support APOE function and overall brain health. Omega-3 fatty acids, found in fatty fish, walnuts, and flaxseeds, have been shown to enhance APOE function and neuroprotective mechanisms.

How does the APOE protein contribute to cardiovascular health?

The APOE protein helps regulate cholesterol levels by facilitating the transportation of cholesterol and lipids. APOE plays a role in removing excess cholesterol from cells and transporting it back to the liver for metabolism and elimination. This process is important for maintaining cardiovascular health.

Are there any lifestyle factors that can influence the expression or impact of the APOE protein?

Yes, lifestyle factors can influence the expression and impact of the APOE protein. Regular physical exercise has been shown to increase APOE expression and enhance its function in lipid metabolism and clearance of amyloid-beta. A healthy diet rich in antioxidants, omega-3 fatty acids, and low in saturated fats may also support APOE function and reduce the risk of neurodegenerative diseases.

Does everyone have the same form of the APOE protein?

No, there are three common forms of the APOE protein known as APOEε2, APOEε3, and APOEε4. Each individual inherits two copies of the APOE gene, resulting in six possible genotypes: APOEε2/ε2, APOEε2/ε3, APOEε2/ε4, APOEε3/ε3, APOEε3/ε4, and APOEε4/ε4.

How does the APOE protein contribute to brain health?

The APOE protein is crucial for various functions in the brain. It assists in the transport and delivery of lipids required for neuronal growth, maintenance, and repair. It also plays a role in clearing away excess amyloid-beta, a protein associated with the formation of plaques in Alzheimer's disease. APOE isoforms have different abilities to clear amyloid-beta, with APOEε4 being less efficient in clearance, contributing to increased Alzheimer's risk.

What other diseases or conditions are associated with the APOE protein?

Apart from Alzheimer's disease and cardiovascular health, variations in the APOE protein have also been linked to other conditions. For example, the APOEε4 allele is associated with an increased risk of developing age-related macular degeneration (AMD), a leading cause of vision loss in older adults. Additionally, APOE genotypes have been implicated in cognitive decline, stroke risk, and traumatic brain injury outcomes.

Are there any ongoing clinical trials targeting the APOE protein?

There are ongoing clinical trials exploring potential interventions targeting the APOE protein. These trials focus on developing drugs or therapies that can modify APOE function, enhance lipid metabolism, reduce amyloid-beta buildup, and improve brain health in conditions such as Alzheimer's disease and other neurodegenerative disorders.

Can lifestyle factors influence the impact of the APOE protein on health?

Yes, lifestyle factors such as diet, exercise, and maintaining a healthy weight can modulate the impact of the APOE protein on health. A healthy lifestyle, including a balanced diet and regular physical activity, may help mitigate the risk of developing certain diseases associated with APOE genotypes, such as Alzheimer's disease or cardiovascular conditions.

Can the APOE protein impact the response to traumatic brain injury?

Yes, the APOE genotype can influence the response to traumatic brain injury (TBI). Studies have shown that individuals carrying the APOEε4 allele may be at an increased risk of poorer outcomes after experiencing a TBI. APOE genotype can influence the severity and recovery process, providing insights into personalized approaches for TBI management and rehabilitation.

Can the APOE protein be therapeutic target in cardiovascular diseases?

The APOE protein has been a target of research for potential therapeutic interventions in cardiovascular diseases. Developing drugs or therapies that modulate APOE function, such as promoting cholesterol efflux or reducing inflammation, might have the potential to improve lipid metabolism and reduce the risk of developing cardiovascular diseases. However, further studies are necessary to develop effective treatments.

How is the APOE protein related to Alzheimer's disease?

Certain variations in the APOE gene, specifically the APOE ε4 allele, have been identified as a major risk factor for developing late-onset Alzheimer's disease. Individuals with one copy of the APOE ε4 allele have an increased risk, while those with two copies have an even higher risk.

Are there any therapies targeting the APOE protein?

While there are no specific therapies directly targeting the APOE protein, research is ongoing to understand its role better in various diseases. Potential therapeutic approaches focus on modulating lipid metabolism, reducing cholesterol deposition, and developing treatments for Alzheimer's disease that target the impact of APOE ε4 allele.

Can the APOE protein impact response to dietary fats?

Yes, the APOE genotype can influence an individual's response to dietary fats. Studies suggest that individuals carrying the APOEε4 allele may be more susceptible to adverse effects of high saturated fat intake, such as elevated cholesterol levels and increased cardiovascular risk.

Are there any potential therapeutic interventions targeting the APOE protein in Alzheimer's disease?

Researchers are actively studying potential therapeutic interventions targeting the APOE protein in Alzheimer's disease. These interventions aim at increasing the clearance of amyloid-beta plaques, reducing brain inflammation, and promoting brain health. However, no specific treatments directly targeting the APOE protein have been approved for clinical use thus far.