Recombinant Mouse B4galt1 overexpression lysate
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
| Cat.No. : | B4galt1-25HCL |
| Product Overview : | Transient overexpression lysate of Mouse UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1) with a C-Myc/DDK tag was expressed in HEK293T. Preparation: HEK293T cells in 10-cm dishes were transiently transfected. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20 centigrade before shipping. Components: 1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 μg empty vector transfected control cell lysate in RIPA buffer 1 vial of 250 μL 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT) |
| Description : | This gene encodes two distinct enzyme isoforms, a long membrane-bound form and a short soluble form. These alternate isoforms are thought to be produced through alternative nested transcription initiation and different in-frame start codon usage. These enzymes catalyze the transfer of galactose to acceptor sugars, such as N-acetylglucosamine and glucose. The long form of this enzyme is localized to the trans-Golgi membrane and is involved in glycoconjugate biosynthesis. The short form functions in lactose biosynthesis though formation of a heterodimer with alpha-lactalbumin. |
| Source : | HEK293T |
| Species : | Mouse |
| Tag : | Myc/DDK |
| Molecular Mass : | 44.4 kDa |
| Stability : | Avoid repeated freeze-thaw cycles. Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. |
| Storage : | Upon receiving, store the sample at -20 centigrade. |
| Reconstitution : | Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20 centigrade for long term storage. |
| Shipping : | The lysate is shipped with dry ice. |
| SDS-PAGE : |
|
| Gene Name : | B4galt1 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 [ Mus musculus (house mouse) ] |
| Official Symbol : | B4galt1 |
| Synonyms : | B4galt1; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1; Ga; GalT; Ggtb; Ggtb2; b4Gal-T1; B-1,4-GalT; B-1,4-GalT1; beta4Gal-T1; beta-1,4-GalT; beta-1,4-GalT1; beta-1,4-galactosyltransferase 1; N-acetyllactosamine synthase; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1; b1,4-Galactosyltransferase I; beta-1,4-GalTase 1; beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; galactosyltransferase 2 beta 1, 4; glycoprotein galactosyltransferase beta 1, 4; lactose synthase A protein; nal synthase; neolactotriaosylceramide beta-1,4-galactosyltransferase; EC 2.4.1.22; EC 2.4.1.275; EC 2.4.1.38; EC 2.4.1.90 |
| Gene ID : | 14595 |
| mRNA Refseq : | NM_022305 |
| Protein Refseq : | NP_071641 |
| UniProt ID : | P15535 |
◆ Recombinant Protein
| B4GALT1-350B | Recombinant Bovine B4GALT1 Protein (1-24 aa), GST-tagged | +Inquiry |
| B4galt1-07HCL | Recombinant Mouse B4galt1 overexpression lysate | +Inquiry |
| B4galt1-1806M | Recombinant Mouse B4galt1 Protein, Myc/DDK-tagged | +Inquiry |
| B4GALT1-2409B | Recombinant Bovine B4GALT1 Protein (1-24 aa), GST-tagged | +Inquiry |
| B4GALT1-646H | Recombinant Human B4GALT1, His tagged | +Inquiry |
|
|
◆ Lysates
| B4GALT1-2532HCL | Recombinant Human B4GALT1 cell lysate | +Inquiry |
|
|
B2MB4GALT1B3GAT1B3GALT2B3GAT3B3GNT2B4GALNT1B4GALT4B3GNT1B3GNT3B4GALT5B3GALT6B3GNT6B4GALT3B4GALT7B-LAB3GNT4B3GNT5B3GALNT1B3GALTLB3GAT2B4GALT6B4GALNT3B4GALT2B9D2B3GALNT2B3galt5B3GNTL1B4GALNT2B9D1B230208H17RikB020004J07RikB020031M17RikB230118H07RikB230120H23RikB230217C12RikB230219D22RikB230312A22RikB630005N14RikB930041F14RikB930007M17RikB3GALT4B4galnt4B430306N03RikB3gnt7B3gnt8B3gnt9
Easy access to products and services you need from our library via powerful searching tools.
Copyright © 2007 – 2021 Creative BioMart. All Rights Reserved. Terms and Conditions | Privacy Policy
