Recombinant Mouse Bmp2 protein, His-tagged

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Recombinant Mouse Bmp2 protein, His-tagged

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Cat.No. : Bmp2-562M
Product Overview : Recombinant Mouse Bmp2 (P21274) aa. (Gln281~Arg394) fused with N-terminal His tag was produced in E. coli cells.
Description : This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. While a homozygous knockout mouse for this gene exhibits embryonic lethality, conditional knockout mice have defects in bone, cartilage and heart development.
Source : E. coli
Species : Mouse
Tag : His
Form : Freeze-dried powder
Molecular Mass : 15kDa as determined by SDS-PAGE reducing conditions
Protein length : Gln281~Arg394
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 97%
Characteristic : The isoelectric point is 8.2.
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200µg/mL
Storage buffer : 10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex
SDS-PAGE :

Gene Name : Bmp2 bone morphogenetic protein 2 [ Mus musculus (house mouse) ]
Official Symbol : Bmp2
Synonyms : Bmp2; bone morphogenetic protein 2; Bmp2a; AI467020; bone morphogenetic protein 2A
Gene ID : 12156
mRNA Refseq : NM_007553.3
Protein Refseq : NP_031579.2
UniProt ID : P21274

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