"Cd320" Related Products


Recombinant Mouse Cd320 Protein, His-tagged

Cat.No.: Cd320-980M
Product Overview: Recombinant mouse CD320 (NP_062294.3) (Met1-Gly208) was expressed with a C-terminal polyhistidine tag.
Source: HEK293
Species: Mouse
Tag: His
Predicted N Terminal: Ala 29
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse CD320-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse TCN2-His, The EC50 of biotinylated mouse TCN2-His is 0.10-0.24 μg/mL.
Molecular Mass: The recombinant mouse CD320 comprises 191 amino acids and has a predicted molecular mass of 21 kDa. The apparent molecular mass of the protein is approximately 47.7 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Cd320 CD320 antigen [ Mus musculus ]
Official Symbol: Cd320
Synonyms: 8D6; NG29; VLDL; TCblR; 425O18-1; D17Ertd716e; putative VLDL lipoprotein receptor; transcobalamin receptor
Gene ID: 54219
mRNA Refseq: NM_001305169
Protein Refseq: NP_001292098
UniProt ID: Q9Z1P5
Chromosome Location: 17 B1; 17 17.98 cM
Pathway: Cobalamin (Cbl, vitamin B12) transport and metabolism, organism-specific biosystem; Metabolism of vitamins and cofactors, organism-specific biosystem; Metabolism of water-soluble vitamins and cofactors, organism-specific biosystem
Function: cobalamin binding; growth factor activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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