Recombinant Mouse CD3D & CD3E protein(Met1-Ala105 & Met1-Asp108), His-Fc&Flag-Fc-tagged
Cat.No. : | CD3D & CD3E-1325M |
Product Overview : | Recombinant Mouse CD3D (P04235)(Met1-Ala105) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant Mouse CD3E (P22646)(Met1-Asp108) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Mouse CD3D/CD3E heterodimer was purified and expressed in HEK293. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Mouse |
Tag : | C-His-Fc&Flag-Fc |
Protein length : | Met1-Ala105 & Met1-Asp108 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Molecular Mass : | The recombinant heterodimer of Mouse CD3D/CD3E comprises 669 (334+335) amino acids and has a calculated molecular mass of 75.4 (37.5+37.9) kDa. As a result of glycosylation, the apparent molecular mass of Mouse CD3D/CD3E heterodimer is approximately 36-55 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 90 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | Cd3d CD3 antigen, delta polypeptide [ Mus musculus ] |
Official Symbol : | CD3D; CD3E |
Synonyms : | CD3D; CD3 antigen, delta polypeptide; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; T3d; |
Gene ID : | 12500 |
mRNA Refseq : | NM_013487 |
Protein Refseq : | NP_038515 |
Products Types
◆ Recombinant Protein | ||
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CD3D-137H | Recombinant Human CD3D Protein, His-tagged | +Inquiry |
CD3D-28H | Active Recombinant Human CD3D Protein (Phe22-Ala105), C-Fc-tagged | +Inquiry |
CD3D-1293H | Recombinant Human CD3D protein(Met1-Ala105), hFc&FLAG-tagged | +Inquiry |
CD3D-136C | Recombinant Cynomolgus monkey CD3D Protein, His-tagged | +Inquiry |
◆ Lysates | ||
CD3D-1466CCL | Recombinant Cynomolgus CD3D cell lysate | +Inquiry |
CD3D-1274HCL | Recombinant Human CD3D cell lysate | +Inquiry |
CD3D-1381MCL | Recombinant Mouse CD3D cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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