Recombinant Mouse Cd3E&D-309M, His-Fc & Flag-Fc tag

Cat.No. : Cd3E&D-309M
Product Overview : A DNA sequence encoding the extracellular domain (Met 1- Ala 105) of mouse CD3D was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1- Asp 108) of mouse CD3E was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
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Cat. No. : Cd3E&D-309M
Description : T-cell surface glycoprotein CD3 delta/epsilon chain, also known as CD3D and CD3E respectively, are single-pass type I membrane proteins. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). The immunofluorescence defined that CD3 epsilon(CD3E0) was strongly stained bound to either CD 3 gamma or CD 3 delta. The recognition by this panel of anti-CD3 antibodies of CD3-gamma/ epsilon and CD3-delta/ epsilon complexes and not of CD3-epsilon alone was assessed by immunoprecipitation.
Source : Human Cells
Molecular Mass : The recombinant heterodimer of mouse CD3D/CD3E comprises 670 (333+337) amino acids and has a calculated molecular mass of 76 (38+38)KDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 45-50 KDa in SDS-PAGE under reducing conditions.
Predicted N terminal : Phe 22& Asp 22
Purity : > 90 % as determined by SDS-PAGE.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Formulation : Lyophilized from sterile PBS, pH 7.4.
Storage : Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : A hardcopy of COA with reconstitution instruction is sent along with the products.
Tag : His&Fc&Flag
Protein length : Met1-Ala105;Met1-Asp108
Synonyms : Cd3e; CD3 antigen, epsilon polypeptide; CD3; T3e; AI504783; CD3epsilon; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3E; T3E; TCRE; Cd3d; CD3 antigen, delta polypeptide; T3d; CD3D; CD3-DELTA; T3D; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain
Synonyms : Cd3e; CD3 antigen, epsilon polypeptide; CD3; T3e; AI504783; CD3epsilon; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3E; T3E; TCRE; Cd3d; CD3 antigen, delta polypeptide; T3d; CD3D; CD3-DELTA; T3D; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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03/22/2023

    Manufacturers with the capacity for bulk production can offer CD3E protein in larger quantities, which is advantageous for researchers working on large-scale experiments or collaborations.

    09/12/2021

      bulk production often leads to economies of scale, making the CD3E protein more cost-effective for research budgets.

      06/17/2018

        Manufacturers can actively seek collaborations and partnerships with researchers to develop new applications or optimize methodologies using CD3E protein.

        Q&As (5)

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        Can CD3E be used to assess the effectiveness of cancer immunotherapies? 04/07/2021

        Yes, CD3E levels and T-cell function can be monitored to evaluate the response to cancer immunotherapies like checkpoint inhibitors.

        Are there any CD3E-targeted therapies? 06/01/2017

        There are currently no approved therapies that directly target CD3E, but it is a target for research in developing immunotherapies and cancer treatments.

        Are there any ongoing clinical trials involving CD3E? 12/20/2016

        There may be ongoing clinical trials using CD3E-targeted therapies in the context of cancer and autoimmune diseases. It's important to check clinical trial databases for specific studies.

        Can CD3E levels be used to monitor the progression of HIV? 08/27/2016

        Yes, monitoring CD3E levels in HIV-infected individuals can provide valuable information about the progression of the disease and the effectiveness of antiretroviral therapy.

        How is CD3E used in allergy diagnostics? 06/22/2016

        CD3E can be used in research to study T-cell responses in allergic conditions, helping to better understand and diagnose allergies.

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