Recombinant Mouse CD79A & CD79B protein(Met1-Arg137 & Met1-Asp158), His-tagged

• Cat.No.: CD79A & CD79B-1152M
• Product Overview: Recombinant Mouse CD79A (NP_031681.2) (Met1-Arg137) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant Mouse CD79B (NP_031681.2) (Met1-Asp158) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the Mouse CD79A & CD79B heterodimer was purified in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Arg137 & Met1-Asp158
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse CD79A & mouse CD79B heterodimer comprises 264 (120+144) amino acids and has a calculated molecular mass of 30.3 (13.8+ 16.5) kDa. The apparent molecular mass of the recombinant protein is approximately 34.6, 28.3 and 24.1 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd79a CD79A antigen (immunoglobulin-associated alpha) [ Mus musculus ]
• Official Symbol: CD79A
• Synonyms: CD79A; CD79A antigen (immunoglobulin-associated alpha); B-cell antigen receptor complex-associated protein alpha chain; Ig alpha; MB-1 membrane glycoprotein; surface IgM-associated protein; immunoglobulin-associated alpha; membrane-bound immunoglobulin-associated protein; Iga; Ly54; mb-1; Ly-54; Igalpha; Ig-alpha
• Gene ID: 12518
• mRNA Refseq: NM_007655
• Protein Refseq: NP_031681