Recombinant Mouse Clp1 Protein, Myc/DDK-tagged

• Cat.No.: Clp1-2196M
• Product Overview: Purified recombinant protein of mouse full-length CLP1, cleavage and polyadenylation factor I subunit (Clp1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Its role in tRNA splicing and maturation is required for cerebellar development. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.
• Molecular Mass: 47.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Clp1 CLP1, cleavage and polyadenylation factor I subunit [ Mus musculus (house mouse) ]
• Official Symbol: Clp1
• Synonyms: Clp1; CLP1, cleavage and polyadenylation factor I subunit; Heab; AI462438; polyribonucleotide 5'-hydroxyl-kinase Clp1; ATP/GTP-binding protein; polynucleotide kinase Clp1; pre-mRNA cleavage complex II protein Clp1; EC 2.7.1.78
• Gene ID: 98985
• mRNA Refseq: NM_133840
• Protein Refseq: NP_598601
• UniProt ID: Q99LI9