Recombinant Mouse Clybl Protein, Myc/DDK-tagged

• Cat.No.: Clybl-2204M
• Product Overview: Purified recombinant protein of mouse full-length citrate lyase beta like (Clybl), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism. Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway. The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite. Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate. Also displays malyl-CoA thioesterase activity. Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. Also has very weak citramalate synthase activity in vitro.
• Molecular Mass: 37.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Clybl citrate lyase beta like [ Mus musculus (house mouse) ]
• Official Symbol: Clybl
• Synonyms: CLYBL; citrate lyase beta like; citrate lyase subunit beta-like protein, mitochondrial; citrate lyase beta-like; Clb; AI256068; 0610033J05Rik; 2310014M14Rik
• Gene ID: 69634
• mRNA Refseq: NM_029556
• Protein Refseq: NP_083832
• UniProt ID: Q8R4N0