Recombinant Mouse CSF3 protein(Met1-Ala208)

• Cat.No.: CSF3-1165M
• Product Overview: Recombinant Mouse CSF3 (NP_034101.1) (Met1-Ala208) was expressed and purified in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: N/A
• Protein length: Met1-Ala208
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 40-160 pg/mL. 2. Immobilized G-CSF Protein, Mouse, Recombinant, HPLC-verified at 2 μg/ml (100 μl/well) can bind CSF3R/G-CSFR Protein, Human, Recombinant (hFc Tag), The EC50 is 7-38 ng/mL.
• Molecular Mass: The recombinant mouse CSF3 consists of 178 amino acids and predicts a molecular mass of 19 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
  > 95 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Csf3 colony stimulating factor 3 (granulocyte) [ Mus musculus ]
• Official Symbol: CSF3
• Synonyms: CSF3; colony stimulating factor 3 (granulocyte); granulocyte colony-stimulating factor; Csfg; G-CSF; MGI-IG
• Gene ID: 12985
• mRNA Refseq: NM_009971
• Protein Refseq: NP_034101