Recombinant Mouse Cth Protein, MYC/DDK-tagged

• Cat.No.: Cth-350M
• Product Overview: Purified recombinant protein of full-length mouse cystathionase (cystathionine gamma-lyase) (Cth), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.
• Molecular Mass: 43.6 kDa
• Purity: >80%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 ug/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cth cystathionase (cystathionine gamma-lyase) [ Mus musculus (house mouse) ]
• Official Symbol: Cth
• Synonyms: Cth; cystathionase (cystathionine gamma-lyase); CSE; Cys3; AI314617; BC019483; 0610010I13Rik; cystathionine gamma-lyase; cysteine-protein sulfhydrase; EC 4.4.1.1
• Gene ID: 107869
• mRNA Refseq: NM_145953
• Protein Refseq: NP_666065
• UniProt ID: Q8VCN5