Recombinant Mouse Ctsa protein(Met1-Tyr474), His-tagged

• Cat.No.: Ctsa-908M
• Product Overview: Recombinant Mouse CTSA (P16675-1) (Met 1-Tyr 474) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Protein length: Met1-Tyr474
• Form: Lyophilized from sterile 25mM Tris, 0.3M NaCl, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant mouse CTSA consists of 462 amino acids and has a calculated molecular mass of 52.8 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ctsa cathepsin A [ Mus musculus ]
• Official Symbol: Ctsa
• Synonyms: CTSA; cathepsin A; lysosomal protective protein; carboxypeptidase C; carboxypeptidase L; protective protein cathepsin A; protective protein for beta-galactosidase; PPCA; Ppgb; AU019505
• Gene ID: 19025
• mRNA Refseq: NM_001038492
• Protein Refseq: NP_001033581

Reviews

06/12/2022

Product arrived quickly, undamaged.

04/04/2022

Great results, worth buying.

01/27/2022

Highly pure, excellent product.

Q&As

Are there any post-translational modifications that regulate CTSA activity?

Post-translational modifications of CTSA are not well-documented, requiring further research for comprehensive understanding.

How does CTSA influence cellular processes outside the lysosome?

CTSA has roles in various cellular processes outside the lysosome, though these are less well understood compared to its lysosomal functions.

What is the significance of CTSA in the degradation of glycoproteins?

CTSA is crucial for the catabolism of glycoproteins, aiding in the removal of terminal sugar moieties.

What therapeutic strategies are being explored to address deficiencies or dysfunctions in CTSA?

Therapeutic strategies for CTSA-related disorders are in the early stages of development, with enzyme replacement therapy being a potential avenue.

How is CTSA dysfunction associated with Galactosialidosis?

Dysfunction or deficiency in CTSA leads to Galactosialidosis, characterized by the accumulation of sialyloligosaccharides and glycoproteins in tissues.

How does CTSA contribute to the stability and activation of other lysosomal enzymes?

It forms a complex with β-galactosidase and neuraminidase, preventing their premature degradation and facilitating their activation.

What is the primary function of CTSA in the lysosome?

CTSA acts as a protective protein, ensuring the stability and proper function of other lysosomal enzymes.