Recombinant Mouse Dgkb Protein, Myc/DDK-tagged

• Cat.No.: Dgkb-2535M
• Product Overview: Purified recombinant protein of mouse full-length diacylglycerol kinase, beta (Dgkb), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable). Has a higher activity with long-chain diacylglycerols like 1,2-di-(9Z-octadecenoyl)-sn-glycerol compared to 1,2-didecanoyl-sn-glycerol. Specifically expressed in brain, it regulates neuron-specific morphological changes including neurite branching and neurite spine formation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 89.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Dgkb diacylglycerol kinase, beta [ Mus musculus (house mouse) ]
• Official Symbol: Dgkb
• Synonyms: DGKB; diacylglycerol kinase, beta; diacylglycerol kinase beta; DAG kinase beta; diglyceride kinase beta; DGK; 90kda; DAGK2; DGK-beta; mKIAA0718; 6430574F24; C630029D13Rik
• Gene ID: 217480
• mRNA Refseq: NM_178681
• Protein Refseq: NP_848796
• UniProt ID: Q6NS52