Recombinant Mouse EEF2 Protein (2-858 aa), His-tagged

• Cat.No.: EEF2-1382M
• Product Overview: Recombinant Mouse EEF2 Protein (2-858 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.

Specification

• Description: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movent of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
• Source: Yeast
• Species: Mouse
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 97.2 kDa
• Protein length: 2-858 aa
• AA Sequence: VNFTVDQIRAIMDKKANIRNMSVIA HVDHGKSTLTDSLVCKAGIIASARA GETRFTDTRKDEQERCITIKSTAIS LFYELSENDLNFIKQSKDGSGFLIN LIDSPGHVDFSSEVTAALRVTDGAL VVVDCVSGVCVQTETVLRQAIAERI KPVLMMNKMDRALLELQLEPEELYQ TFQRIVENVNVIISTYGEGESGPMG NIMIDPVLGTVGFGSGLHGWAFTLK QFAEMYVAKFAAKGEGQLSAAERAK KVEDMMKKLWGDRYFDPANGKFSKS ANSPDGKKLPRTFCQLILDPIFKVF DAIMNFRKEETAKLIEKLDIKLDSE DKDKEGKPLLKAVMRRWLPAGDALL QMITIHLPSPVTAQKYRCELLYEGP PDDEAAMGIKSCDPKGPLMMYISKM VPTSDKGRFYAFGRVFSGVVSTGLK VRIMGPNYTPGKKEDLYLKPIQRTI LMMGRYVEPIEDVPCGNIVGLVGVD QFLVKTGTITTFEHAHNMRVMKFSV SPVVRVAVEAKNPADLPKLVEGLKR LAKSDPMVQCIIEESGEHIIAGAGE LHLEICLKDLEEDHACIPIKKSDPV VSYRETVSEESNVLCLSKSPNKHNR LYMKARPFPDGLAEDIDKGEVSARQ ELKARARYLAEKYEWDVAEARKIWC FGPDGTGPNILTDITKGVQYLNEIK DSVVAGFQWATKEGALCEENMRGVR FDVHDVTLHADAIHRGGGQIIPTAR RCLYASVLTAQPRLMEPIYLVEIQC PEQVVGGIYGVLNRKRGHVFEESQV AGTPMFVVKAYLPVNESFGFTADLR SNTGGQAFPQCVFDHWQILPGDPFD NSSRPSQVVAETRKRKGLKEGIPAL DNFLDKL
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: Eef2 eukaryotic translation elongation factor 2 [ Mus musculus ]
• Official Symbol: EEF2
• Synonyms: EEF2; elongation factor 2; Ef-2; MGC98463
• Gene ID: 13629
• mRNA Refseq: NM_007907
• Protein Refseq: NP_031933
• UniProt ID: P58252