Recombinant Mouse Engase Protein, Myc/DDK-tagged

• Cat.No.: Engase-2824M
• Product Overview: Purified recombinant protein of mouse full-length endo-beta-N-acetylglucosaminidase (Engase), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Endoglycosidase that releases N-glycans from glycoproteins by cleaving the beta-1,4-glycosidic bond in the N,N'-diacetylchitobiose core. Involved in the processing of free oligosaccharides in the cytosol.
• Molecular Mass: 82.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Engase endo-beta-N-acetylglucosaminidase [ Mus musculus (house mouse) ]
• Official Symbol: Engase
• Synonyms: Engase; endo-beta-N-acetylglucosaminidase; C130099A03; D230014K01Rik; cytosolic endo-beta-N-acetylglucosaminidase; EC 3.2.1.96
• Gene ID: 217364
• mRNA Refseq: NM_172573
• Protein Refseq: NP_766161
• UniProt ID: Q8BX80