Recombinant Mouse EphB1 protein(Met1-Leu539), His-tagged

• Cat.No.: Ephb1-910M
• Product Overview: Recombinant Mouse EPHB1 (Q8CBF3-1) (Met 1-Leu 539) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-539 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized mouse EPHB1-His at 10 μg/ml (100 μl/well) can bind mouse EFNB1-Fc, The EC50 of mouse EFNB1-Fc is 0.04-0.08 μg/ml.
• Molecular Mass: The secreted recombinant mouse EPHB1 consists of 533 amino acids and has a predicted molecular mass of 59.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm EPHB1 is approximately 64 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ephb1 Eph receptor B1 [ Mus musculus ]
• Official Symbol: EphB1
• Synonyms: EPHB1; Eph receptor B1; Elk
• Gene ID: 13843