Recombinant Mouse Ephx2 Protein, His-tagged

• Cat.No.: Ephx2-36M
• Product Overview: Recombinant mouse Ephx2 protein with a N-terminal His-tag was expressed in Sf21 cells.

Specification

• Description: Mammalian soluble epoxide hydrolase (sEH) is a member of the α/β-hydrolase fold family of enzymes that catalyzes the hydrolysis of exogenous and endogenous epoxides to vicinal diols. sEH is a homodimer consisting of two domains. The C-terminal domain is responsible for the epoxide hydrolase activity while the N-terminal domain has a catalytic center with phosphatase activity. Endogenous substrates for sEH include epoxyeicosatrienoic acids (EETs) which are known for their vasodialatory effects as well as for their anti-inflammatory actions. Inhibition of sEH in animal models was shown to reduce hypertension and vascular inflammation, as well as related syndromes, validating sEH as a highly promising target for drug discovery.
• Source: Insect cells
• Species: Mouse
• Tag: His
• Molecular Mass: 64 kDa
• Purity: ≥95% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmole of 6-methoxy-2-naphthaldehyde per minute at 37 centigrade in 25 mM bis-Tris, pH 7.0, containing 1 μM PHOME.
• Stability: ≥ 6 months
• Storage: Store it at -80 centigrade
• Storage Buffer: TBS buffer at pH 7.4 with 20% glycerol

Gene Information

• Gene Name: Ephx2 epoxide hydrolase 2, cytoplasmic [ Mus musculus (house mouse) ]
• Official Symbol: Ephx2
• Synonyms: Ephx2; epoxide hydrolase 2, cytoplasmic; CEH; SEH; sEP; Eph2; bifunctional epoxide hydrolase 2; cytosolic epoxide hydrolase; epoxide hydratase; epoxide hydrolase 2C; soluble epoxide hydrolase
• Gene ID: 13850
• mRNA Refseq: NM_001271403
• Protein Refseq: NP_001258332
• UniProt ID: P34914