Recombinant Mouse Ephx2 Protein, His-tagged Ephx2-36M

Recombinant Mouse Ephx2 Protein, His-tagged


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Recombinant Mouse Ephx2 Protein, His-tagged

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Cat.No. : Ephx2-36M
Product Overview : Recombinant mouse Ephx2 protein with a N-terminal His-tag was expressed in Sf21 cells.
Description : Mammalian soluble epoxide hydrolase (sEH) is a member of the α/β-hydrolase fold family of enzymes that catalyzes the hydrolysis of exogenous and endogenous epoxides to vicinal diols. sEH is a homodimer consisting of two domains. The C-terminal domain is responsible for the epoxide hydrolase activity while the N-terminal domain has a catalytic center with phosphatase activity. Endogenous substrates for sEH include epoxyeicosatrienoic acids (EETs) which are known for their vasodialatory effects as well as for their anti-inflammatory actions. Inhibition of sEH in animal models was shown to reduce hypertension and vascular inflammation, as well as related syndromes, validating sEH as a highly promising target for drug discovery.
Source : Insect cells
Species : Mouse
Tag : His
Molecular Mass : 64 kDa
Purity : ≥95% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 nmole of 6-methoxy-2-naphthaldehyde per minute at 37 centigrade in 25 mM bis-Tris, pH 7.0, containing 1 μM PHOME.
Stability : ≥ 6 months
Storage : Store it at -80 centigrade
Storage Buffer : TBS buffer at pH 7.4 with 20% glycerol
SDS-PAGE Analysis of Ephx2 :

Lane 1: MW Standards; Lane 2: Mouse sEH (2 μg); Lane 3: Mouse sEH (4 μg). Representative gel image shown; actual purity may vary between each batch.

Figure :

Gene Name : Ephx2 epoxide hydrolase 2, cytoplasmic [ Mus musculus (house mouse) ]
Official Symbol : Ephx2
Synonyms : Ephx2; epoxide hydrolase 2, cytoplasmic; CEH; SEH; sEP; Eph2; bifunctional epoxide hydrolase 2; cytosolic epoxide hydrolase; epoxide hydratase; epoxide hydrolase 2C; soluble epoxide hydrolase
Gene ID : 13850
mRNA Refseq : NM_001271403
Protein Refseq : NP_001258332
UniProt ID : P34914

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