Recombinant Mouse Epo protein, His & S-tagged

• Cat.No.: Epo-603M
• Product Overview: Recombinant Mouse Epo (B7ZMY9) aa. (Val82~Arg192) fused with N-terminal His & S tag was produced in E. coli cells.

Specification

• Description: This gene encodes the glycoprotein hormone erythropoietin that regulates the production of red blood cells and biosynthesis of hemoglobin. The predominant expression of this gene shifts from the liver during fetal development to kidney in adults. A complete lack of the encoded protein causes embryonic lethal anemia in mice. The conditional inactivation of this gene in adult mice results in a chronic, normocytic and normochromic anemia. Transgenic mice expressing the human ortholog of this gene exhibit polycythemia. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: E. coli
• Species: Mouse
• Tag: His & S
• Form: Freeze-dried powder
• Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions
• Protein length: Val82~Arg192 (Accession # P07321)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 6.2.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Dilutions: PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4

Gene Information

• Gene Name: Epo erythropoietin [ Mus musculus (house mouse) ]
• Official Symbol: Epo
• Synonyms: Epo; erythropoietin
• Gene ID: 13856
• mRNA Refseq: NM_001312875.1
• Protein Refseq: NP_001299804.1
• UniProt ID: B7ZMY9

Reviews

12/28/2021

Stability is truly remarkable, can maintain its activity even under extreme conditions.

08/31/2021

Can catalyze reactions even under extreme conditions.

02/03/2019

This protein's repeatability is truly exceptional, consistently delivering reliable results even under challenging conditions.

Q&As

What is the relationship between EPO protein and cardiovascular disease?

EPO protein can promote the renewal and repair of heart blood vessels, and has the effect of protecting the heart from ischemia-reperfusion damage.

How does EPO protein regulate erythropoiesis?

By binding to receptors, EPO proteins activate signaling pathways that promote the proliferation and differentiation of hematopoietic stem cells in bone marrow into erythrocytes.

How about the clinical use of EPO protein?

EPO protein can be used by injection or oral, and the specific method and dosage need to be determined according to the condition and the doctor's advice.

What are the implications of EPO protein research for the future of treating anemia?

The study of EPO protein helps to understand the regulatory mechanism of erythropoiesis and provides new ideas and methods for the treatment of anemia, such as developing more effective EPO protein analogues or improving existing treatment regimens.

How is EPO protein treated for anemia?

This protein stimulates the bone marrow to produce more red blood cells, thereby increasing hemoglobin levels and treating anemia.

What is the role of EPO protein in kidney disease?

In kidney disease, due to impaired kidney function, the production of EPO protein decreases, leading to anemia. Supplementation with exogenous EPO protein can help correct anemia caused by kidney problems.