Recombinant Mouse Eri1 Protein, Myc/DDK-tagged

• Cat.No.: Eri1-2859M
• Product Overview: Purified recombinant protein of mouse full-length exoribonuclease 1 (Eri1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Binds with high affinity to the 3' side of the stem-loop structure and to the downstream cleavage product (DCP) of histone pre-mRNAs. Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 39.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Eri1 exoribonuclease 1 [ Mus musculus (house mouse) ]
• Official Symbol: Eri1
• Synonyms: ERI1; exoribonuclease 1; 3-5 exoribonuclease 1; eri-1 homolog; 3-5 exonuclease ERI1; enhanced RNAi protein 1; histone mRNA 3-exonuclease 1; three prime histone mRNA exonuclease 1; histone mRNA 3 end-specific exonuclease; Thex1; eri-1; 3hexo; 3110010F15Rik
• Gene ID: 67276
• mRNA Refseq: NM_026067
• Protein Refseq: NP_080343
• UniProt ID: Q7TMF2