Recombinant Mouse Ero1b Protein, Full Length, C-Flag tagged

• Cat.No.: Ero1b-042M
• Product Overview: Purified full length recombinant protein of Mouse ERO1-like beta (S. cerevisiae) (Ero1lb), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Flag
• Protein Length: Full Length
• Description: Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2, PDIA3, PDIA4, PDIA6 and NXNDC12. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell (By similarity). Involved in oxidative proinsulin folding in pancreatic cells, hence required for glucose homeostasis in vivo.
• Molecular Mass: 53.5 kDa
• AA Sequence: MSPGFRRAVTGQGAAAAVQLLVTLSFLSSLVKTQVTGVLDDCLCDIDSIDKFNTYKIFPKIKKLQERDYFRYYKVNLKRPCPFWAEDGHCSIKDCHVEPCPESKIPVGIKAGRSNKYSQAANSTKELDDCEQANKLGAINSTLSNESKEAFIDWARYDDSQDHFCELDDERSPAAQYVDLLLNPERYTGYKGSSAWRVWNSIYEENCFKPRSVYRPLNPLAPSRGEDDGESFYTWLEGLCLEKRVFYKLISGLHASINLHLCANYLLEETWGKPSWGPNIKEFRRRFDPVETKGEGPRRLKNLYFLYLIELRALSKVAPYFERSIVDLYTGNVEDDADTKTLLLSIFQDTKSFPMHFDEKSMFAGDKKGAKSLKEEFRLHFKNISRIMDCVGCDKCRLWGKLQTQGLGTALKILFSEKEIQNLPENSPSKGFQLTRQEIVALLNAFGRLSTSIRELQNFKALLQHRRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Notes: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >0.05 μg/μL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol

Gene Information

• Gene Name: Ero1b endoplasmic reticulum oxidoreductase 1 beta [ Mus musculus (house mouse) ]
• Official Symbol: Ero1b
• Synonyms: Ero1b; endoplasmic reticulum oxidoreductase 1 beta; Ero1lb; ero1-beta; 1300013B24Rik; 1700065B09Rik; ERO1-like protein beta; ERO1-L-beta; ERO1-like beta; endoplasmic oxidoreductase 1 beta; endoplasmic oxidoreductin-1-like protein B; endoplasmic reticulum oxidoreductase beta; endoplasmic reticulum oxidoreductin-1-like protein B; oxidoreductin-1-L-beta
• Gene ID: 67475
• mRNA Refseq: NM_026184
• Protein Refseq: NP_080460
• UniProt ID: Q8R2E9