Recombinant Mouse F10 protein, His-tagged

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Recombinant Mouse F10 protein, His-tagged

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Cat.No. : F10-2461M
Product Overview : Recombinant Mouse F10 protein (Gly21-Asn481) was fused to His-tag at N-terminus and expressed in E.coli.
Description : This gene encodes factor X, a component of both the intrinsic and extrinsic blood coagulation pathways. The encoded protein is a zymogen that undergoes further processing in a vitamin K-dependent manner to generate mature factor X, a heterodimer comprised of disulfide-linked heavy and light chains. The mature factor X is proteolytically activated either by factor IXa (intrinsic pathway) or factor VIIa (extrinsic pathway) to form factor Xa serine endopeptidase. Activated factor Xa catalyzes the conversion of prothrombin to thrombin. A complete lack of the encoded protein is fatal to mice. A severe deficiency of the encoded protein in mice causes age-dependent iron deposition and cardiac fibrosis. Alternative splicing results in multiple transcript variants encoding different isoforms.
Source : E.coli
Species : Mouse
Tag : His
Form : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Molecular Mass : 56 kDa as determined by SDS-PAGE reducing conditions.
Protein length : 461
Endotoxin : <1.0 EU per 1µg (determined by the LAL method).
Purity : >97%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
SDS-PAGE of F10-2461M :

Gene Name : F10
Official Symbol : F10
Synonyms : fX; Cf10; FX; FXA; Stuart Prower Factor; Thrombokinase
Gene ID : 14058
mRNA Refseq : NM_007972.4
Protein Refseq : NP_031998.3
UniProt ID : O88947

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