Recombinant Mouse Fars2 Protein, Myc/DDK-tagged

• Cat.No.: Fars2-2955M
• Product Overview: Purified recombinant protein of mouse full-length phenylalanine-tRNA synthetase 2 (mitochondrial) (Fars2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 52.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Fars2 phenylalanine-tRNA synthetase 2 (mitochondrial) [ Mus musculus (house mouse) ]
• Official Symbol: Fars2
• Synonyms: FARS2; phenylalanine-tRNA synthetase 2 (mitochondrial); phenylalanine--tRNA ligase, mitochondrial; pheRS; phenylalanyl-tRNA synthetase, mitochondrial; phenylalanine-tRNA synthetase 1 (mitochondrial); Fars1; 2810431B21Rik; 6720478K01Rik
• Gene ID: 69955
• mRNA Refseq: NM_024274
• Protein Refseq: NP_077236
• UniProt ID: Q99M01