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Recombinant Mouse Fcgrt/B2m protein, His/Strep II/Avi-tagged, Biotinylated

Cat.No.: Fcgrt & B2m-1546M
Product Overview: Recombinant Mouse FCGRT(Ser 22 - Ser 297)/B2M(Ile 21 - Met 119), the subunit FCGRT / FcRN carries an Avi tag at the C-terminus, followed by a polyhistidine tag, the subunit Beta-2 microglobulin (B2M) carries a Twin Strep tag at the C-terminus was expressed in HEK293.
Source: HEK293
Species: Mouse
Tag: His/Strep II/Avi
Predicted N Terminal: Ser 22 (FcRN) & Ile 21 (B2M)
Form: Lyophilized from 0.22um filtered solution in PBS (pH 7.4) with Trehalose as protectant.
Molecular Mass: The protein has a calculated MW of 34.7 kDa (FcRN), 14.7 kDa (B2M) . The protein migrates as 45-50 kDa (FCGRT), 15 kDa (B2M) respectively on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Protein length: FCGRT(Ser 22 - Ser 297)/B2M(Ile 21 - Met 119)
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Notes: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.No activity loss is observed after storage at:4-8 centigrade for 12 months in lyophilized state;-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 200 μg/mL in sterile deionized water. For best performance, we strongly recommend you to follow the reconstitution protocol provided in the COA.
Gene Name: Fcgrt Fc receptor, IgG, alpha chain transporter [ Mus musculus ]
Official Symbol: Fcgrt
Synonyms: FCGRT; Fc receptor, IgG, alpha chain transporter; IgG receptor FcRn large subunit p51; neonatal Fc receptor; igG Fc fragment receptor transporter alpha chain; FcRn;
Gene ID: 14132
mRNA Refseq: NM_010189
Protein Refseq: NP_034319
MIM:
UniProt ID: Q61559
Chromosome Location: 7 B4; 7 29.12 cM
Function: IgG binding; beta-2-microglobulin binding; receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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