Recombinant Mouse Fchsd2 Protein, Myc/DDK-tagged

• Cat.No.: Fchsd2-2977M
• Product Overview: Purified recombinant protein of mouse full-length FCH and double SH3 domains 2 (Fchsd2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Adapter protein that plays a role in endocytosis via clathrin-coated pits. Contributes to the internalization of cell surface receptors, such as integrin ITGB1 and transferrin receptor. Promotes endocytosis of EGFR in cancer cells, and thereby contributes to the down-regulation of EGFR signaling. Recruited to clathrin-coated pits during a mid-to-late stage of assembly, where it is required for normal progress from U-shaped intermediate stage pits to terminal, omega-shaped pits. Binds to membranes enriched in phosphatidylinositol 3,4-bisphosphate or phosphatidylinositol 3,4,5-trisphosphate. When bound to membranes, promotes actin polymerization via its interaction with WAS and/or WASL which leads to the activation of the Arp2/3 complex. Does not promote actin polymerisation in the absence of membranes.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 82.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Fchsd2 FCH and double SH3 domains 2 [ Mus musculus (house mouse) ]
• Official Symbol: Fchsd2
• Synonyms: Fchsd2; FCH and double SH3 domains 2; NWK1; Sh3md; R74866; Sh3md3; BC034086; mKIAA0769
• Gene ID: 207278
• mRNA Refseq: NM_199012
• Protein Refseq: NP_950177
• UniProt ID: Q3USJ8