Recombinant Mouse Gde1 Protein, Myc/DDK-tagged

• Cat.No.: Gde1-3181M
• Product Overview: Purified recombinant protein of mouse full-length glycerophosphodiester phosphodiesterase 1 (Gde1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Hydrolyzes the phosphodiester bond of glycerophosphodiesters such as glycerophosphoinositol (GroPIns) and glycerophosphoethanolamine (GroPEth), to yield a glycerol phosphate and an alcohol. Hydrolyzes glycerophospho-N-acylethanolamines to N-acylethanolamines in the brain and partipates to bioactive N-acylethanolamines biosynthesis such as anandamide (an endocannabinoid), N-palmitoylethanolamine (an anti-inflammatory), and N-oleoylethanolamine (an anorexic). In addition, has a lysophospholipase D activity by hydrolyzing N-acyl-lysoplasmenylethanolamine (N-acyl-lysoPlsEt) to N-acylethanolamine. However lysophospholipase D activity is lower than glycerophosphodiester phosphodiesterase activity. Has little or no activity towards glycerophosphocholine.
• Molecular Mass: 38.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gde1 glycerophosphodiester phosphodiesterase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Gde1
• Synonyms: GDE1; glycerophosphodiester phosphodiesterase 1; membrane interacting protein of RGS16; membrane-interacting protein of RGS16; Mir16; RGS16; 1200003M13Rik
• Gene ID: 56209
• mRNA Refseq: NM_019580
• Protein Refseq: NP_062526
• UniProt ID: Q9JL56