Active Recombinant Mouse Gh protein, His-tagged

• Cat.No.: Gh-783M
• Product Overview: Active Recombinant Mouse Gh protein(P06880)(28~216 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 28-216 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Growth hormone (GH), also known as somatotropin is a peptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans an do ther animals. It is a type of mitogen which is specific only to certain kinds of cells. GHplay a major role in body growth by stimulating the liver and other tissuestosecrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid up take and protein synthesis in musclean do ther tissues. Besides, Growth Hormone Receptor (GHR) has been identifiedasan interactor of GH, thus a binding ELISA assay was conducted to detect theinteraction of recombinant mouse GH and recombinant mouse GHR. Briefly, GHwere diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uLwere then transferred to GHR-coated microtiter wells and incubated for 2hat 37°C. Wells were washed with PBST and incubated for 1h with anti-GHpAb, then aspirated and washed 3 times. After incubation with HRP labelled secondaryantibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of GH and GHR was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 25.4kDa
• Purity: > 97% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Gh growth hormone [ Mus musculus ]
• Official Symbol: Gh
• Synonyms: GH; growth hormone; somatotropin; Gh1
• Gene ID: 14599
• mRNA Refseq: NM_008117
• Protein Refseq: NP_032143