Recombinant Mouse Grem1 Protein

• Cat.No.: Grem1-7380M
• Product Overview: Recombinant mouse Gremlin-1 / GREM1(aa. 160) without tag was expressed in E. coli.

Specification

• Description: Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro). Acts as inhibitor of monocyte chemotaxis.
• Source: E. coli
• Species: Mouse
• Form: Lyophilized
• Molecular Mass: 28 kDa
• AA Sequence: MKKKGSQGAIPPPDKAQHNDSEQTQ SPPQPGSRTRGRGQGRGTAMPGEEV LESSQEALHVTERKYLKRDWCKTQP LKQTIHEEGCNSRTIINRFCYGQCN SFYIPRHIRKEEGSFQSCSFCKPKK FTMMVTLNCPELQPPTKKKRVTRVK QCRCISIDLD
• Endotoxin: < 0.1 ng/μg of Grem1
• Purity: > 95 % by SDS-PAGE and Silver staining
• Stability: Shelf life: one year from despatch.
• Storage: The lyophilized protein is stable at room temperature for up to 1 week or at -20 centigrade for longer. Following reconstitution store (in aliquots) at -20 centigrade. Avoid repeated freezing and thawing.
• Storage Buffer: 50 mM acetic acid without stabilizer
• Reconstitution: Mouse Grem1 should be reconstituted in 50 mM acetic acid or water to a concentration of 0.1 mg/mL. This solution can be diluted in water or other buffer solutions.

Gene Information

• Gene Name: Grem1 gremlin 1, DAN family BMP antagonist [ Mus musculus (house mouse) ]
• Official Symbol: Grem1
• Synonyms: Grem1; gremlin 1, DAN family BMP antagonist; Dr; ld; Drm; gre; Grem; Cktsf1b; Cktsf1b1; gremlin-1; cysteine knot superfamily 1, BMP antagonist 1; down-regulated in Mos-transformed cells protein; down-regulated in v-mos-transformed cells
• Gene ID: 23892
• mRNA Refseq: NM_011824
• Protein Refseq: NP_035954
• UniProt ID: O70326