Recombinant Mouse Hectd3 Protein, Myc/DDK-tagged

• Cat.No.: Hectd3-3379M
• Product Overview: Purified recombinant protein of mouse full-length HECT domain E3 ubiquitin protein ligase 3 (Hectd3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus facilitating cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8.
• Molecular Mass: 97.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Hectd3 HECT domain E3 ubiquitin protein ligase 3 [ Mus musculus (house mouse) ]
• Official Symbol: Hectd3
• Synonyms: HECTD3; HECT domain containing 3; E3 ubiquitin-protein ligase HECTD3; HECT domain-containing protein 3; probable E3 ubiquitin-protein ligase HECTD3; AI467540; AW743312; RP23-419D6.4; 1700064K09Rik
• Gene ID: 76608
• mRNA Refseq: NM_175244
• Protein Refseq: NP_780453
• UniProt ID: Q3U487